SDS-PAGE/CoomassiebluestainingPARP1&PARP1Automodified
●PARP1Automodified(PARylated),humanPoly(ADP-ribose)polymerase-1(PARP1)isanabundantandubiquitousnuclearenzymethatcatalyzestheNAD(+)-dependentadditionofADP-ribosepolymersonavarietyofnuclearproteins. TheTulipBiolabs,Inc. PARP1Automodified,Cat.#2095,ishighlypurifiedhumanPARP1,expressedinabaculovirusexpressionsystem,towhichNAD+ andcofactorshavebeenaddedtoallowPARPautomodificationbypoly(ADP-ribose).ThePARP1islyophilizedfromitsnativestate,anduponreconstitutionwithwater,itretainsresidualpolyADP-ribosylationenzymaticactivity.
●UseinWesternBlot,100ng/laneusingHRP/TMBcolorimetricmethods●ELISA,2ng/well
●SDS-PAGE,0.75µg/lane●Asastandardinotherassays
Pleaseseethe Cat.#2095Datasheet(pdf) foracompletedescription.