IsolationfromMDCKCells:PARP1andTnks1
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IsolationofPARsylatedPARP1
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●Af1521MacrodomainPoly- andmono-ADP-riboseAffinityResinSetTheTulipBiolabs,Inc.Af1521MacrodomainAffinityResinSetisdesignedfortheisolationofpoly-ADP-ribosylated(PARylated)andmono-ADP-ribosylated(MARylated)proteinsfromculturedcellsandtissuesamples.Throughtheuseofahighlyspecificmacrodomainaffinityresin,poly-ADP-ribosylated(PARylated)andmono-ADP-ribosylated(MARylated)proteinsareisolatedfromcellortissuelysates. Theresinboundproteinscanbeelutedfromtheaffinityresin,andanalyzedbyimmunoblotting,massspec,orothermethods.
Cat.#4301Setcontains1eachofthefollowing(canbeorderedseparately):
•Af1521MacrodomainAffinityResin,1mg macrodomainfusionproteinsuppliedasaslurrycontaining ~75µLresin,Cat.#2302
•Af1521MacrodomainNegativeControlResin,0.5mgfusionproteinsuppliedasaslurrycontaining~75µLresin, Cat.#2303
TheAf1521MacrodomainAffinityResin,Cat.#2302ishighlypurifiedGST-Af1521macrodomainfusionproteinconstructexpressedinE.coli,andboundtoglutathionebeads. TheAf1521macrodomainproteinbindspoly-andmono-ADP-ribosylatedproteins,pluspoly(ADP-ribose)andfreeADP-ribose.
PARNegativeControlResin,Cat.#2303isidenticaltothe#2302resinexceptfortwoglytoaspsubstitutions,whichabolishbinding. Thenegativecontrolresinisusefultocontrolfornon-specificbinding,anditsuseisoptional.
Pleaseseethe Cat.#4301 Datasheet(pdf) foracompletedescription.
●Af1521MacrodomainAffinityMagneticResinNEW! TheTulipBiolabs,Inc.Af1521Macrodomainisnowavailableasamagneticresin,Cat.#2305. ThisisthesamemacrodomainproteincapableofbindingPARylatedandMARylatedproteinsasCat.#2302excepthereitissuppliedboundtosuperparamagneticbeadsforeasierhandlingandhigherthroughput!
Pleaseseethe Cat.#2305 Datasheet(pdf) foracompletedescription.
