IsolationfromHEK293Cells:PARP1andTnks1 

<clickimagetoenlarge>

●RNF146WWEAffinityResinSetTheTulipBiolabs,Inc.RNF146WWEAffinityResinSetisdesignedfortheisolationofpoly-ADP-ribosylatedproteinsfromculturedcellsandtissuesamples.ThroughtheuseofahighlyspecificWWEaffinityresin,poly-ADP-ribosylated(PARylated)proteinsareisolatedfromcellortissuelysateswithouttheuseofanti-PARantibodies. Theresinboundproteinscanbeelutedfromtheaffinityresin,andanalyzedbyimmunoblottingorothermethods.

Cat.#4306Setcontains1eachofthefollowing(canbeorderedseparately):

•WWEResin,0.5mgWWEfusionproteinsuppliedasaslurrycontaining ~50µLresin,Cat.#2334 

•WWENegControlResin,0.5mgfusionproteinsuppliedasaslurrycontaining~50µLresin, Cat.#2335

TheWWEAffinityResin,Cat.#2334ishighlypurifiedGST-RNF146(100-175)fusionproteinconstructexpressedinE.coli,andboundtoglutathionebeads. 

WWENegativeControlResin,Cat.#2335isidenticaltothe#2334resinexceptforR163Asubstitution,whicheffectivelyabolishesPARbinding. Thenegativecontrolresinisusefultocontrolfornon-specificbinding,anditsuseisoptional.

Pleaseseethe Cat.#4306 Datasheet(pdf) foracompletedescription.